Studies on growth and cannabinoid composition of callus derived of Cannabis sativa
Publication 6
Lloydia, Vol. 41, No. 5, Sep-Oct, 1978
John K. Hemphill, Jocelyn C. Turner & Paul G. Mahlberg
Department of Biology, Indiana University, Bloomington, IN 47401
Keywords: Cannabis sativa, Cannabaceae, Ultrastructural, Capitate, Glandular, Hairs
Abstract
Callus derived from different plant parts of various strains of Cannabis Sativa L. was established and analyzed for growth on different media and for cannabinoid content by thin-layer (tic) and gas-liquid chromatography (glc). Plant organs which included bracts and anther-calyx complexes of drug (152) and fiber (150) types and maturing leaves of Mexican (drug) and Turkish (fiber) strains were shown to accumulate characteristic cannabinoids and, therefore, were selected as callus source material. Callus initiated from these plant organs demonstrated different growth responses to casein hydrolysate (CH) which were different from its growth response to various amino acid mixtures as media supplements. Numerous roots were formed on newly initiated callus and subcultured tissues of several transfers. Addition of 2,4- dichlorophenoxyacetic acid at 0.2 mg/liter inhibited root formation. In long-term cultures, different concentrations of naphthaleneacetic acid and kinetin did not initiate root and/or shoot differentiation. However, growth responses of these callus strains differed from each other when maintained on various combinations of these growth regulators. Under these experimental conditions, no major cannabinoids were detected in callus extracts, although numerous unknown metabolites were present. One minor cannabinoid was detected as indicated from a positive Fast Blue Salt B test.
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