A temporal study of cannabinoid composition in continual clones of Cannabis sativa L. (Cannabaceae)
Publication 19
Ann F. Vogelmann,
Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California 91109
Jocelyn C. Turner & Paul G. Mahlberg.
Department of Biology, Indiana University, Bloomington, Indiana 47405
Keywords: Cannabis sativa, Cannabaceae, cannabinoids, seedling, leaf length
Abstract
Seedlings of a ,D9-tetrahydrocannabinol (THC) dominant strain of Cannabis sativa were employed to develop a biological system to investigate cannabinoid biosynthesis. High-performance liquid chromatography was used to detect and quantify the cannabinoids. Cannabinoids were first detected in seedlings of light/dark-grown plants at 48-50 h. The first cannabinoid detected was cannabichromene (CBC) at 52-54 h. At 60-62 h both cannabigerol (CBG) and THC were detected along with CBC. A similar sequence appeared in dark-grown seedlings, but CBC became evident only at 56-58 h, and THC and CBG were first detected at 66-68 h. This pattern is significant because previously proposed pathways of cannabinoid synthesis have indicated that CBG is a precursor to CBC, yet CBG is not the first cannabinoid to be detected but occurs later with the appearance of THC. Cannabinoid concentrations were always higher in light-grown than in dark-grown plants of comparable age. Cannabinoid quantities on a dry-weight basis increased with increasing leaf length and/or increasing age.
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