Thin-layer chromatographic identification of phenol in the glandular secretory system of Cannabis sativa L. (Cannabaceae)
Publication 23
Proceedings of the Indiana Academy of Science (1988) Volume 98 p. 109-116.
Charles T. Hammond
Department of Biology
St. Meinrad College
St. Meinrad, Indiana 47577
and
Paul G. Mahlberg
Department of Biology
Indiana University
Bloomington, Indiana 47405
Keywords: Cannabis sativa, Cannabaceae, thin layer, chromatography, phenol
Abstract
A phenolic glycoside was isolated from vegetative stem exudates from a Mexican strain of Cannabis and tentatively identified by thin layer chromatography (TLC) as phloroglucinol-glucoside. Incubation at 100°C for 30 min in 2N HCl liberated a free phenol which was identified by mass spectroscopy and 1 H-nuclear magnetic resonance as phloroglucinol. Glandular heads were isolated in large numbers with high purity from floral regions by blending, microfiltration and centrifugation in cold. Gland samples extracted for phenols as above revealed by TLC the presence of phloroglucinol. Phloroglucinol may play an important role in the in vivo biosynthesis of cannabinoids.
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